mouse anti tlr2  (Proteintech)

Journal: Frontiers in Immunology

Article Title: Macrophage IL-1β turns meningeal fibroblasts into inflammatory amplifiers in pneumococcal infection

doi: 10.3389/fimmu.2026.1808185

Figure Lengend Snippet: Pneumococcal challenge in human CSF generated higher release of LDH, IL-6 and IL-8 by Ben-Men-1 cells. Ben-Men-1 cells were challenged with increasing concentrations of SP D39 (0.25/0.5/1/2x10 7 CFU/ml) while being held in human CSF from 8 different donors (n=3-6, control: MCM) for 24h. Negative control was pure bacterial THY growth medium. Supernatants were analyzed for LDH (positive control: triton 1%), IL-6 and IL-8 (A, C) . (B) = Summary of data from (A–C) . During pneumococcal challenge (2x10 7 CFU/ml, MCM, negative controls: THY medium, PBS), cells were treated with the following inhibitors: TLR-cocktail (25µg/ml anti-TLR2 antibody + 5µM TLR4 inhibitor + 20µg/ml chloroquine), 0.1-10 µM BAY 11-7082, 0.01-1µM 666-15, 0.01-1µM JQ1 or DMSO control. (D) Supernatants were analyzed for LDH and IL-6. Data are given as individual values as well as means ± SD.

Article Snippet: In addition, TLR signaling was assessed using an anti-TLR2 mAB T2.5 (25μg/ml, Hycult), TLR4 inhibitor TAK-242 (5μM, Calbiochem, USA) and chloroquine (20μg/ml, Sigma-Aldrich, USA) to inhibit endosomal TLRs ( ).

Techniques: Generated, Control, Negative Control, Positive Control

Journal: Frontiers in Immunology

Article Title: Macrophage IL-1β turns meningeal fibroblasts into inflammatory amplifiers in pneumococcal infection

doi: 10.3389/fimmu.2026.1808185

Figure Lengend Snippet: Primary human meningeal cells display partially PLY-dependent cell death and cytokine release upon pneumococcal challenge. HMC were challenged with P/S-lysed S. pneumoniae serotype 2 (D39), D39Δ ply , serotypes 6B and 7F (0.25/1/4x10 7 CFU/ml, negative control: THY bacterial medium without bacteria) for 24h. Supernatants were analyzed for LDH (C, D) and IL-6 release (B, E) After exposure to THY, D39 or D39Δ ply (4x10 7 CFU/ml), supernatants were pooled and analyzed with antibody array. (A) HMC were treated during pneumococcal challenge (4x10 7 CFU/ml) with the following inhibitors: TLR-cocktail (anti-TLR2 antibody T2.5 (25µg/ml) + TLR4 inhibitor TAK-242 (5µM) + endosomal TLR inhibitor chloroquine (20µg/ml)), NF-κB inhibitors parthenolide (0.5µM) and BAY 11-7082 (1-10 µM), AP-1 inhibitor SR11302 (1µM), BRD4 inhibitors I-BET (10µM) and JQ1 (1µM), CREB inhibitors KG-501 (10µM) and 666-15 (1µM), or DMSO control. (D, E) Data are given as individual values as well as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Statistical analysis was calculated with ANOVA with Tukey’s multiple comparison test.

Article Snippet: In addition, TLR signaling was assessed using an anti-TLR2 mAB T2.5 (25μg/ml, Hycult), TLR4 inhibitor TAK-242 (5μM, Calbiochem, USA) and chloroquine (20μg/ml, Sigma-Aldrich, USA) to inhibit endosomal TLRs ( ).

Techniques: Negative Control, Bacteria, Ab Array, Control, Comparison

Journal: iScience

Article Title: cGAS/STING sensing in dendritic cells discriminates between daptomycin sensitive and resistant Staphylococcus aureus clinical isolates

doi: 10.1016/j.isci.2026.115854

Figure Lengend Snippet: TLR2 is required for MutuDC sensing of, but not internalization of MRSA (A) Relative pHrodo labeled MRSA internalization by MutuDC over 4 h following stimulation with DapS A8819 (light blue symbols) or DapR A8817 (dark blue symbols), or media alone (white squares). Prior to stimulation, MutuDC were pre-treated for 1 h with TLR2 blocking antibody (clone T2.5; triangles with dashed lines) or media alone (circles with filled lines). Relative MRSA internalization by each DC subset is expressed as the gMFI of pHrodo. Results show the mean and (SD) of duplicates from one experiment, representative of two independent experiments. (B) Cytokine secretion (pg/mL) by MutuDC stimulated with TLR2 ligand peptidoglycan of S. aureus (PGN-SA) (10 μg/mL) or (C) DapS (A8819; light blue) or DapR (A8817; dark blue) MRSA (MOI of 10) for 18 h. MutuDC were first pre-treated with either TLR2 blocking antibody (dot-filled bars) or media alone (filled bars) as in A, or an isotype control (clone 163D3, empty bars) at 1 μg/mL. Results pooled from four (B) or three (C) independent experiments and expressed as the mean ± SEM, with each symbol (circle, square, and directional triangles) representing paired experimental replicates ( n = 3). Statistical significance determined using paired t test and reported as indicated by an ∗ when p ≤ 0.05. (D) Expression of surface activation markers by MutuDC stimulated with DapS A8819 MRSA. DC were pre-treated with TLR2 blocking antibody (black trace), isotype control (dashed red trace), and media alone (light blue shaded). Unstained control sample is shown for each marker (black dashed trace). Data shown from one experiment, representative of three independent experiments.

Article Snippet: mAB mTLR2- anti-mouse/human TLR2 , InvivoGen , Cat# mab-mtlr2; RRID: AB_763722.

Techniques: Labeling, Blocking Assay, Control, Expressing, Activation Assay, Marker, FACS

Journal: iScience

Article Title: cGAS/STING sensing in dendritic cells discriminates between daptomycin sensitive and resistant Staphylococcus aureus clinical isolates

doi: 10.1016/j.isci.2026.115854

Figure Lengend Snippet:

Article Snippet: mAB mTLR2- anti-mouse/human TLR2 , InvivoGen , Cat# mab-mtlr2; RRID: AB_763722.

Techniques: Purification, Cell-Signaling, Enzyme-linked Immunosorbent Assay, Western Blot, Virus, Recombinant, Transfection, Derivative Assay, Expressing, Clone Assay, Plasmid Preparation, Software